Goat anti-Human IgA, IgG, and

Goat anti-Human IgA, IgG, and IgM, Alkaline Phosphatase conjugate

IgG is a monomeric immunoglobulin, built of two heavy chains and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells.

Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B-cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA.

Specific for human IgG, IgM and IgA.

• ELISA
• Western Blotting

Western Blot:
1:5,000-1:50,000 dilution can be used.

Optimal working dilutions must be determined by the end user.

Human

Human

IgG

Purified goat secondary antibody IgG in lyophilized buffer containing 0.01 M Tris-HCL, 0.25 M NaCl, pH 8.0 with 15 mg/mL BSA and 0.05% sodium azide.

Store lyophilized material at 2-8°C for up to 1 year after date of receipt.
After reconstitution this product is stable for several weeks at 2-8°C as an undiluted liquid. DO NOT FREEZE. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade or better) to make a final concentration of 50% glycerol and store at 2-8°C for up to 12 months.
Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.

Routinely evaluated by Enzyme-linked Immunosorbent Assay.

ELISA:
1:5,000-1:50,000 diltution can be used.

Secondary & Control Antibodies

Whole Immunoglobulin Secondary Antibodies

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Chemicon (Millipore)

Alkaline Phosphatase

Goat

Polyclonal Antibody