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- 详细信息
- 文献和实验
- 技术资料
- 库存:
18
- 英文名:
2-(Diphenylphosphino)ethylamine
- CAS号:
4848-43-5
- 保质期:
2年
- 供应商:
上海联迈生物工程有限公司
- 保存条件:
2-8℃
- 规格:
1g
- 英文名:
- 2-(Diphenylphosphino)ethylamine
- 别名:
- CAS号:
- 4848-43-5
- 分子式:
- C14H16NP
- 分子量:
- 229.26
- MDL:
- MFCD00233831

- 敏感性: 易吸潮
- 储存条件: 2-8℃
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文献和实验Labeling Muscle Actin with 5-iodoacetamidofluorescein
with the dialysis buffer and read the OD at 495 nm. D/P = {OD495 x 41 / 60,000} / {(mg/ml)/ 43,000},should be 0.5-0.9. 3.Dilute to 3-5 mg/ml with the dialysis buffer.Calculate total mg of actin.Store as aliquots in liquid N2 after dissolving 2 mg sucrose per mg
Labeling Muscle Actin with 5-iodoacetamidofluorescein
/protein molar ratio. Dilute 1:40 with the dialysis buffer and read the OD at 495 nm. D/P = {OD495 x 41 / 60,000} / {(mg/ml) / 43,000}, should be 0.5-0.9. 3. Dilute to 3-5 mg/ml with the dialysis buffer. Calculate total mg of actin. Store
LABELING MUSCLE ACTIN WITH IATR
1.Centrifuge in a 50Ti (1 hr,40,000 rpm)or 42.2Ti (30 min,25,000 rpm)at 4℃. 2.Measure concentration and dye/protein molar ratio.Dilute 1:40 with the dialysis buffer and read the OD at 555 nm. D/P = {OD555 x 41 / 60,000} / {(mg/ml)/ 43,000
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