- 询价
- STEMCELL Technologies
- 05001/05021/05022
- 2026年06月06日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
概要
PneumaCult™-ALI Medium (Catalog #05001) is a serum- and BPE-free medium for the culture of human airway epithelial cells at the air-liquid interface (ALI). Airway epithelial cells cultured in PneumaCult™-ALI Medium undergo extensive mucociliary differentiation to form a pseudostratified epithelium that exhibits morphological and functional characteristics similar to those of the human airway in vivo. PneumaCult™-ALI Medium is also available in a kit that includes 12 mm Transwell® inserts (Catalog #05021) or 6.5 mm Transwell® inserts (Catalog #05022).Together, PneumaCult™-ALI Medium and PneumaCult™-Ex Plus Medium (Catalog #05040) constitute a fully integrated BPE-free culture system for in vitro human airway modeling. This robust and defined system is a valuable tool for basic respiratory research, toxicity studies, and drug development.
Advantages:
• HBECs cultured with PneumaCult™-ALI undergo extensive mucociliary differentiation to form a pseudostratified epithelium that closely resembles the human airway
• PneumaCult™-ALI is serum-free and BPE-free to minimize variability
Components:
- PneumaCult™-ALI Basal Medium, 450 mL
- PneumaCult™-ALI 10X Supplement, 50 mL
- PneumaCult™-ALI Maintenance Supplement (100X), 5 x 1 mL
Cell Type:
呼吸道细胞
Species:
人
Formulation:
无血清
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验利用 Millicell 培养小室培养皮肤及肺类器官的实验方案
http://dx.doi.org/10.1007/978-1-4939-2013-6_7 5.Wilson J. 1992. Epithelial-specific gene expression during differentiation of stratified primary human keratinocyte cultures. Cell Growth Differ. 3(8):471-483. 2.气液界面 (ALI) 3D 培养人支气管上皮细胞 气液界面 （ALI）3D
3 分线外挣扎的你，看别人如何登顶 Nature、Science
的上皮表面。结果：细菌数量少时，non-CF 猪的可清除，CF 猪的感染 (Fig. 1i)；细菌数量多时，两者都感染。以上，ASL 有快速杀菌能力，CF 此能力受损。杀伤缺陷可能源于 ASL 中抗菌物质数量的减少或功能的受阻。知识点呼吸道上皮细胞气液界面（air-liquid interface,ALI）培养。本文用这个培养体系在体外模拟气道上皮产生的 ASL 用于下一步的研究。图：人气道上皮在半透膜上培养，投射电镜下的显微结构。上层空气，ASL 位于其余上皮之间。上皮结构分化出纤毛（→
胶产品。 用于3D细胞培养的dECM肺水凝胶 图2. NHBE原代细胞培养。正常人支气管上皮(NHBE)原代细胞在dECM肺水凝胶或Matrigel®基质中培养10天。在dECM水凝胶中培养的NHBE细胞稳健表达正常肺上皮细胞标志物FOXJ1、KTR5、NKX2.1、panKT、p63和ZO-1(A,B,C)，生成的p63+人支气管上皮细胞亚群(D)比Matrigel®基质更多。 图3. NHBE原代细胞气液界面培养。正常人支气管上皮(NHBE)原代细胞气液界面培养(ALI)21天
