- 询价
- AB3623
- 2025年12月30日
- Western Blotting;Immunohistochemistry
- Rabbit
- Human;Mouse;Rat
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- 文献和实验
- 技术资料
- 应用范围：
Western Blotting;Immunohistochemistry
- 宿主：
Rabbit
- 适应物种：
Human;Mouse;Rat
- 库存：
大量
- 抗原来源：
0
- 是否单克隆：
2
- 规格：
50 μg
Product Images
Lane 1: Untreated non-apoptotic HeLa cell extract . Lane 2: HeLa cells treated with Camptothecin (apoptotic).
Rabbit anti-Caspase 3, Active (Catalog Number AB3623). Localization of Active Caspase (red) in trimethyltin (TMT) treated mouse brain (dentate gyrus (...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IF, IH, IH(P), WB | Rabbit | Affinity Purified | Polyclonal Antibody |
- Apopain
- Yama Protein
- CASP3
- SCA-1
- procaspase3
- Immunofluorescence
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
1:100-1:200 (0.5-4 µg/mL) dilution of active apoptotic cells we used to detect Caspase-3.
Note when using tissue, levels of active caspase 3 can below detectable levels. Use CC119 (human caspase 3) incubated 30 minutes with 1 mM ATP (for activation) as a positive control on westerns. Alternatively, neuronal culture preparations incubated with staurosporine (0.5 µM, 10-18 hr) typically give a strong caspase 3 signal.
Immunohistochemistry:
1:10 dilution (10-20 µg/mL), AB3623 has reacted successfully in 4% PFA fixed, paraffin embedded tissues mouse tissues.
Immunohistochemistry(paraffin):
Optimal Staining of Caspase-3 Monoclonal Antibody: Mouse Small Intestine
- Human
- Mouse
- Rat
- CASP3
- CPP-32
- Yama
- CASP-3
- CPP32B
- SCA-1
- CPP32
- EC 3.4.22.56 [Contains: Caspase-3 subunit p17 Caspase-3 subunit p12].
- Apoptosis & Cancer
- Metabolism
- Caspases
- Enzymes & Biochemistry
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Cleavage specific synthetic peptide sequence (proprietary) 详细描述见链接：http://www.millipore.com/catalogue/item/AB3623
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文献和实验【求助】求论文一篇---Nature：阿尔茨海默病发病机制研究
fragmentation. DR6 is activated locally by an inactive surface ligand(s) that is released in an active form after trophic-factor deprivation, and we identify APP as a DR6 ligand. Trophic-factor deprivation triggers the shedding of surface APP in a -secretase
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
Detection of ERK1/2 Activities Using Affinity Reagents
) using specific anti-ERK1/2 or anti-p38α antibodies and protein A-Sepharose and subjected to immune complex in vitro kinase assays in the presence of equimolar amounts of GST-PTP-SL 147–288 (2 βg; lanes 1 and 3) or MBP (0.85 μg; lanes 2 and 4). ( B ) COS
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