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MilliSolve™ Filtration SystemThe MilliSolve Filtration System features automatic, continuous solvent and buffer filtration-no need to pour during filtration. the 2 L receiving flask includes plug and tubing for storage-eliminates filtered buffer transfer to reduce labor and contamination risk. the conical 2 L flask bottom allows access to all filtered solvent/buffer. |
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详细描述见链接:http://www.millipore.com/catalogue/item/XX1604700
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文献和实验Plant DNA methylation analysis by bisulfite genomic sequencing
DNA clean up resin (Promega kit #A7280), vortex (see comment 4); Load resin on provided column, apply vacuum (vacuum flask, membrane pump); Add 2.0ml 80% (v/v) 2-propanol on column, apply vacuum (vacuum flask, membrane pump);
Whole genome amplification protocol for ChIP-chip
to a genomic tiling array. Therefore, we have adapted the standard protocol for whole genome amplification using the Sigma GenomePlex WGA kit to amplify our ChIP sample (O'Geen et al., 2006). Using Oct4 ChIP-chip assays as an example, we have compared
Endothelial Cell Systems - Instructions For Use
area of flask x Recommended Seeding Density Example: 600,000 viable cells / 75 cm2 x 2500 cells/cm2 = 3 T-75 flasks (rounded down to nearest whole number) Use the following equation to calculate the volume of cell suspension to seed
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