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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
ELISA;Western Blotting
- 宿主:
Rabbit
- 库存:
大量
- 抗原来源:
0
- 适应物种:
Rat
- 是否单克隆:
2
- 规格:
50 μg
A 20 amino acid peptide sequence near the N-terminus of rat AE1 (Kudrycki et al. 1989; Kopito et al. 1985; Raida et al. 1989; Lux et al. 1989; Tanner et al. 1988). 详细描述见链接:http://www.millipore.com/catalogue/item/AB3500P
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文献和实验Dual- and Triple-Color Fluorospot
stimulatory agents of choice and anti-CD28 (0.1 μg/ml; seeNote 4), in a final volume of 100–150 μl/well. The number of cells per well is dependent on the stimulating agent used (seeNote 5). b. Incubate the plate overnight at 37°C in a humidified
required to kill 50% of J774A.1 cells (50% effective concentration [EC50]) when they were incubated with PA (1 μg/ml) at 37°C (Table 1). The protein was measured by the method described by Lowry et al.. By using this procedure, rLF was purified
and pre-elution bead equilibration. 4.Bind sample for 2 hours 5.Elute four times with 1mg/ml of flag.Expect ~50% of the TFC4 in the WCE to bind to the beads.Almost all of the TFC4 bound to the beads will elute in the first 3 elutions. Anion Exchange
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