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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Western Blotting
- 宿主:
Rabbit
- 抗原来源:
0
- 库存:
大量
- 适应物种:
Canine;Human;Mouse;Monkey
- 是否单克隆:
2
- 规格:
100 μL
synthetic peptide corresponding to amino acids 287-304 of canine ZO-1 associated nucleic acid-binding protein (ZONAB) 详细描述见链接:http://www.millipore.com/catalogue/item/07-684
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文献和实验Testing Rabbit Bleeds By Elisa
antibody diluted in Blocking Buffer for 2 hr at RT. I do tripling dilutions beginning at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP
Polyclonal Antibody Production
For injection of two rabbits, your antigen should be in 1 mL of a buffer such as PBS or Tris that contains no chemicals harmful to the rabbit. 100 µg/rabbit of antigen is best, but you can use less and get good results.
IMMUNOSCREENING OF WITH ANTIBODY PROBES
Adsroption of Anti-E. coli Antibodies Grow E. coli strain Y1090 in L broth at 37°C overnight. Transfer to 10 mM MgSO4 and store at 4°C for future use. These cells are good for about 1 week. Soak one 132 mm nitrocellulose membrane per Petri dish
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