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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Western Blotting;Immunohistochemistry
- 宿主:
Rabbit
- 适应物种:
Human;Rat
- 库存:
大量
- 抗原来源:
0
- 是否单克隆:
2
- 规格:
50 μL
Product Images
Rabbit anti-BDNF (Catalog Number AB1779) staining of rat spinal cord.
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IH, INHIB | Rabbit | Serum | Polyclonal Antibody |
SPECIES REACTIVITIES: Rat and human. Other species not yet tested. It is likely that the antibody will also work on mouse and porcine due to sequence homology.
- Western Blotting
- Immunohistochemistry
- Inhibits Activity/Function
Immunoblotting:1:1,000; we recommend preparing the BDNF samples for western blot by the method of Katoh-Semba (1997) because BDNF is not easily extracted in standard buffers. Dissected tissues should be homogenized in 10 volumes of 100mM phosphate buffer containing 1mM EDTA, 2M guanidine hydrochloride (pH7.2), and three protease inhibitors, 10mM N-ethylmaleimide, 0.36mM pepstatin, and 1mM PMSF. Homogenates are then sonicated and centrifuged at 46K x g for 30 minutes at 4C.
BIOLOGICAL ACTIVITY: Neutralizes BDNF, but not other neurotrophins.
Optimal working dilutions must be determined by end user.
- Human
- Rat
- BDNF
- Abrineurin
- neurotrophin
- MGC34632
- Neurochemistry & Neurotrophins
- Neuroinflammation & Pain
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
Recombinant Human Brain Derived Neurotrophic Factor (BDNF). 详细描述见链接:http://www.millipore.com/catalogue/item/AB1779
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