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Protein Purification
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Vantage L Chromatography Laboratory ColumnsVantage-L Laboratory Columns provide advanced design for the increased demands of biochromatography. Five diameters and three lengths provide a range of column volumes and bed heights to select from, with the twin adjusters providing 250 mm adjustment in all columns. |
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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
as before; discard supernatant and resuspend as before; recentrifuge and discard supernatant 7.In cold room or on ice, suspend pelleted cells in 2 x 1 mL 10% glycerol; combine fractions and split into 100 µL aliquots 8.If you are not going to use
E L I S A w i t h p l a t e l e t s
PBS-PF (paraformaldehyde), 2% PBS-tween 20, 0.05% glycine (x1) - BSA, 1 mg/ml revelation solution (citrate/phosphate buffer), pH 5, 1 L, water solution = citrique acid, 5.1065 g + Na2 HPO4 x 2H2 0, 9.08 g
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