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Minicon® and Miniplus® ConcentratorsConcentrate urine and cerebrospinal fluid to intensify proteins that indicate abnormal or pathological states prior to analysis by electrophoresis or immunoelectrophoresis (e.g., Bence Jones proteins in urine). |
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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
during apoptosis. The tests were performed on sections of rat, chichen, frog and fish thymus. All the thymus were fixed in Bouin's fixative and embedded in paraffin wax (3). A) Immunocytochemical procedure The low molecular weight DNA fragments
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. x. Place 50 µL of the biotin-avidin-HRP mix on the preparation and cover with a coverslip. Incubate the slides for 40 min at room temperature in a humid chamber. xi. Wash slides in wash solution 1 for 15 min
Bromodeoxyuridine Immunohistochemistry
endogenous peroxidases in a staining dish. 5. 1XPBS, 4 X 5 min., RT 6. Pepsin/HCI, 20 min. (try 30 - 40 min.), RT in a humidifies chamber. Prepare 200 mg/ml pepsin, 0.01N HCI in PBS [from 10 mg/ml pepsin prepared fresh in ddH2O(50x) and 2N HCI (200
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