24-Well Plate, TC-Treated, 50

Layered plates

to spread sample/agar mix and allow to set Pipette or pour 2 mL of soft agar onto plate and swirl to spread. Allow to set. Incubate plates Materials YPD Soft Agar In a 2L flask add: 10g Yeast Extract

Dropout plates for yeast

of the plate, and then just a bit more after that. Recap each plate upon pouring. If there are lots of bubbles in your plates (i.e., more than one or two on the edge), you can flame the plate using the small bunsen burner to eliminate bubbles

Yeast Plates

-agar (Difco) Autoclave 25 min. Add: 10ml (10/mg/ml) of any other required amino acids 10ml 0.4% Adenine sulfate in 0.1M HCl if required 10 ml 0.2% Uracil if required 50 ml 40% glucose   YPD Plates (1 liter): Yeast Extract