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文献和实验Clone Genes From a Phage Library
). Its complexity is roughly 80,000 X an insert size of 4 to 8 kb = 320 Mbp, which is about 20-fold coverage of this yeast genome. The aim is to evenly plate out about 10,000 phage over about 10 plates. What you need in addition to the phage is: Plates: LB
The ribonuclease protection assay (RPA)
The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant RNA polymerases from the bacteriophages SP6, T
A Guide to Yeast Two-Hybrid Experiments（酵母双杂交实验指南）
*Correspondence: rfinley@wayne.edu Introduction The yeast two-hybrid system is a powerful genetic assay for protein-protein interactions (Chien et al., 1991; Fields and Song, 1989). The system has been used in thousands of studies
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