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- Solarbio
- 北京
- T1640
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
T-Vector Zero pTOPO Cloning Kit
- 库存：
99
- 供应商：
北京索莱宝科技有限公司
- 规格：
20T
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文献和实验Cloning vector to 1ml of the Taq-amplified PCR product2. Incubate for 5 minutes on your bench top3. Transform One Shot® Competent E. coli.Topo平端克隆方法和长PCR片断克隆法（Zero Blunt Topo PCR Cloning Kit）（Topo XL PCR Cloning Kit）因为越来越多高确限度热稳定酶如Pfx、Pfu被用于PCR扩增，使到PCR
Nature of the InsertThe cloning of PCR-amplified fragments into a linear vector is typically a rapid and efficient process. However, not all PCR fragments will clone with the same efficiency into the same vector. These differences
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
-end ligation (1 ). The Invitrogen (Carlsbad, CA) TA Cloning� Kit combines the efficiency of cohesive-end ligation with the ease of direct cloning of PCR products. A sample may be removed from the completed PCR reaction and transferred directly
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