- 询价
- IBA
- 德国
- 2-5030-010
- 2026年04月23日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
4-8°C
- 保质期：
6
- 英文名：
Strep-Tactin®XT 4Flow® high capacity 50% suspension
- 库存：
999+
- 供应商：
IBA Lifesciences
- 规格：
20 ml（2-5030-010）/4 ml （2-5030-002）/50 ml （2-5030-025）
| 规格： | 20 ml（2-5030-010） | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 4 ml （2-5030-002） | 产品价格： | 询价 |
| 规格： | 50 ml （2-5030-025） | 产品价格： | 询价 |
Strep-Tactin®XT 4Flow® high capacity is a 4% agarose coupled with the streptavidin variant Strep-Tactin®XT and applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommend for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose enables the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In comparison to Strep-Tactin®XT 4Flow®, Strep-Tactin®XT 4Flow® high capacity has a higher density of immobilized Strep-Tactin®XT, which leads to a 2-fold higher yield of purified protein.
The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.
Specifications
| Bead Size: | 50 - 150 µm spherical |
|---|---|
| Dynamic Protein Binding Capacity: | 14 mg/ml resin |
| Form: | 50% suspension |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Specificity: | Twin-Strep-tag® and Strep-tag®II |
| Support: | 4% agarose, crosslinked |
Shipping information
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
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- 作者
- 内容
- 询问日期
文献和实验[1] Zimmann F, McNicoll F, Thakur P K, et al. Retinitis pigmentosa-linked mutations impair the snRNA unwinding activity of SNRNP200 and reduce pre-mRNA binding of PRPF8[J]. Cellular and Molecular Life Sciences, 2025, 82(1): 103.
[2] Claff T, Ebenhoch R, Kley J T, et al. Structural basis for lipid-mediated activation of G protein-coupled receptor GPR55[J]. Nature Communications, 2025, 16(1): 1973.
[3] Liu C, Park Y J, Ma C B, et al. ACE2 utilization of HKU25 clade MERS-related coronaviruses with broad geographic distribution[J]. bioRxiv, 2025: 2025.02. 19.639017.
Expi Supernatant 进行培养。 纯化过程中，选择柱体积为 0.2 ml 的三种纯化柱：Ni Sepharose 6 FastFlow, Ni Sepharose Excel, 和 Strep-Tactin®XT 4Flow®。 分别用其纯化 60CV (column volume, 柱体积) 和 120CV 的样品，并保持上样样品中的蛋白质含量在纯化柱最大结合载量 80%-95 %。 二、实验结果 01 对比蛋白质回收效率 对于不同的填料，可以从洗脱组分中回
。图 A图 A：分别向 Strep-Tactin®XT High Capacity 1ml 重力柱（左）和 Ni-NTA 1ml 重力柱（右）中，同时加入含 4mg mCherry-Twin-Strep-tag (TST) 或 mCherry-His-tag (His) 萤光蛋白的 ExpiCHO 细胞培养上清（注: 接下来的实验皆是取哺乳细胞表达某抗体后的上清，另加入高纯度目标蛋白进行实验)。由图可见，Strep-Tactin®XT（左）能高效率的捕捉 mCherry-TST，而右方
（IBA Lifesciences, Cat. no.: 6-6201-004），內含：4x Fab-TACS® Gravity column (capacity pro column: 15 ml whole blood)1x CD3 Fab-Strep, human1x Buffer CI (10x)1x Biotin stock solution1x Fab-TACS® Gravity AdapterFab-TACS® Flow Restrictor, Pack of 5(2) Fab-TACS
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