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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Kinase Assay
- 宿主:
0
- 库存:
大量
- 抗原来源:
0
- 是否单克隆:
0
- 规格:
1 mL
- Inhibitors
- Tools for Enzyme Studies
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/20-116
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文献和实验sequence of the GSK-3 and CDK kinase domains (19) . Accordingly, numerous CDK inhibitors are very potent GSK-3 inhibitors (19) . Recently, AR-A014418 has been reported asan ATP-competitive, potent, and specific inhibitor of GSK-3as determined by in vitro
CO-IMMUNOPRECIPITATION ANALYSIS OF PROTEIN-PROTEIN INTERACTIONS
PMSF 0.5% protease inhibitor cocktail (Sigma) pipet cells up and down extensively and then sit on ice for 10 minutes. Spin down cell extracts at max speed for 10 minutes at 40 C. Pool extracts from each transfection
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
buffer: 50 mM Tris-HCl, pH 7.5, 1% (v/v) TritonX-100, 0.27 M sucrose, 1 mM EDTA, 0.1 mM EGTA, 1 mMsodium orthovanadate, 50 mM sodium fluoride, 5 mM sodiumpyrophosphate, 0.1% (v/v) b-mercaptoethanol, and completeprotease inhibitor cocktail (Roche, Basel
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