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文献和实验Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer
of most protein tyrosine phosphatases) Protease inhibitor cocktail (proprietary mixture) 1X PBS Cell scraper 1.5 ml microcentrifuge tube Centrifuge Pipette man Filtered pipette tips Kimwipes Waste beaker
【资源】western blot 磷酸化蛋白检测裂解液完整配方
50ul(2.5M) 100ul 10uM ALLN 5ul(10mM) 10ul 10uM ALLM 5ul(10mM) 10ul 0.1mM Sodium Orthovanadate 5ul(100mM) 10ul Protease Inhibitor Cocktail 5ul 10ul Phosphatase Inhibiror Cocktail 5ul 10ul Complete Tablet 1/2 1(mini
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
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