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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
ELISA;Western Blotting
- 宿主:
0
- 抗原来源:
0
- 库存:
大量
- 是否单克隆:
0
- 规格:
1 mg
- ELISA
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
- ELISA Assays
- Immunohistochemistry Assays
- ELISA Reagents
- Immunohistochemistry Reagents
- Western Blotting Reagents
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/18-152
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文献和实验Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8oC. Dialyze against 3 changed of PBS, changing every 2 hours, 100X the conjugate
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8o C
-MG ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值, b 2-MG 浓度与 OD 值成正比,可通过绘制标准曲线求出标本中 b 2-MG 浓度。 试剂盒组成 ( 2-8℃ 保存) 酶标 板( Coated Wells ) 96 孔 酶标
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