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文献和实验, laminin, and proteoglycans)but also matrix degrading enzymes/their inhibitors and growth factors. Invasion of tumor cells into Matrigel has been used to characterize involvement of ECM receptors and matrix degrading enzymes which play roles in tumor
In Vitro Matrigel Angiogenesis Assays
activity. The method is based on the differentiation of ECs on a basement membrane matrix, Matrigel, derived from the Engelbreth-Holm-Swarm tumor (1 ). ECs from human umbilical cords as well as from other sources differentiate and form capillary
In Vitro Matrigel Fibroblast Invasion Assay
. In this invasion assay the FLS grow through an artificial matrix composed mainly of collagen IV. First, the walls of transwells are coated with paraffin to avoid meniscus formation. Subsequently, the bottoms of the transwells (on top of the membrane) are coated
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