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Goat anti-Rabbit IgG, HRP conj

ugate; 500 μg
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  • 询价
  • 12-348
  • 2025年08月25日
  • ELISA;Western Blotting
  • Goat
  • Rabbit
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      ELISA;Western Blotting

    • 宿主

      Goat

    • 库存

      大量

    • 抗原来源

      0

    • 适应物种

      Rabbit

    • 是否单克隆

      2

    • 规格

      500 μg

    Species Reactivity Key Applications Host Format Antibody Type
    Rb WB, IH, ELISA Goat HRP Polyclonal Antibody
    Description:
    Goat Anti-Rabbit IgG, HRP-conjugate
    Background Information:
    Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
    Specificity:
    Specific for rabbit IgG heavy and light chains.
    Key Applications:
    • Western Blotting
    • Immunohistochemistry
    • ELISA
    Species Reactivity:
    Rabbit
    Species Reactivity Note:
    Rabbit.
    Isotype:
    IgG
    Immunogen:
    Highly purified whole rabbit IgG.
    Presentation:
    Goat polyclonal conjugated with horseradish peroxidase IgG in buffer containing 50% storage buffer 0.02 M potassium phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, 0.01% (w/v) gentamicin sulfate and 50% glycerol.
    Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.
    Storage Conditions:
    Stable for 1 year at from date of receipt.
    Quality Assurance:
    Evaluated by western blot.

    Western Blot Analysis: Suitable for western blotting (dot blot), ELISA and immunohistochemistry. A working dilution of 1:15,000 to 1:60,000 is suggested for ELISA. A working dilution of 1:2,000 to 1:6,000 is suggested for western blotting procedures. A working dilution of 1:500 to 1:3,000 is suggested for immunohistochemistry. Optimal antibody dilution for other applications should be determined by the researcher.
    Antibody Category:
    Secondary & Control Antibodies
    Antibody Sub-Category:
    Whole Immunoglobulin Secondary Antibodies
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Upstate (Millipore)
    Format:
    HRP
    Host:
    Goat
    Concentration:
    1 mg/mL
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    详细描述见链接:http://www.millipore.com/catalogue/item/12-348

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    图标文献和实验
    相关实验
    • 【交流】谁用过RD公司的HRP酶标羊抗鼠IgG试剂盒.

      我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06

    • Anti-P-CREB

      which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates

    • Anti-HA Immunocytology

      -purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)  

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