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- VPVH20CN1
- 2025年07月12日
- Protein Purification
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- 文献和实验
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- 应用范围：
Protein Purification
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Viresolve Process Area ModulesViresolve process area modules are self-contained, single-use disposable modules designed to remove viruses from protein solutions at process scale. Viresolve modules are fabricated from a single material of construction, polyvinylidene fluoride (PVDF). Each unit is 100% integrity tested during manufacturing by CorrTest to assure integrity. |
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详细描述见链接：http://www.millipore.com/catalogue/item/VPVH20CN1
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文献和实验Purification of Antiserum or Ascites by Protein A/G Chromatography
in case the antibody was eluted.Ⅳ After washing the column, collect a 20 microliter fraction and test for protein by adding it to 180 microliters of Coomassie Blue reagent to test for protein. If the sample turns blue, wash the column with an additional 50
cfuge at top speed. Transfer supernatant to polyallomer tubes and centrifuge 1.5 hr at 27,000 rpm at 4' in SW 28.1 rotor (have to divide into 3 tubes). (See also alternate procedure.) C. Resuspend phage pellet in 180 141 5OmM Tris, ph
Purification of DNA from lambda phage
tube,extract with phenol again.You can do a third phenol extraction if there is still a lot of white denatured protein at the interface. E.Add 200 III CHCOC3,shake,microcentrifuge briefly.Transfer aqueous layer to new tube,repeat. F.Add 20 jil of 3M
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