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文献和实验4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
In almost all aerobic cells, the oxygen metabolism generates reactive oxygen species (ROS), such as superoxide, hydroxyl radicals, and hydrogen peroxide. These ROS can peroxidize membrane lipids of a cell and its organelles
) and 4-hydroxy-2-nonenal (HNE) (3 ), a major product of lipid peroxidation and the most reactive of these compounds under physiological conditions (4 ). This fluorescent compound, characterized as a2-hydroxy-3-imino-1,2-dihydropyrrol derivative (3, R1 = R2
Detection of 4-Hydroxy-2-Nonenal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach
diseases. And the methods to identify specific proteins that are susceptible to 4-hydroxy 2-nonenal (HNE) and 3-nitrotyrosine (NT) modifications are limited and difficult. Our laboratory uses two-dimensional polyacrylamide gel electrophoresis (2DE
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