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Anti-Histone H2B; 200 μg

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  • 询价
  • 07-371
  • 2025年08月08日
  • Chromatin Immunoprecipitation
  • Rabbit
  • Chicken;Human;Xenopus
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      Chromatin Immunoprecipitation

    • 宿主

      Rabbit

    • 适应物种

      Chicken;Human;Xenopus

    • 库存

      大量

    • 抗原来源

      0

    • 是否单克隆

      2

    • 规格

      200 μg

    Product Images

    WB: Recombinant Xenopus Histone H2B (lane 1), chicken core histones (lane 2) and acid extract from either sodium butyrate (lane 3) or colcemid treated...

    Species Reactivity Key Applications Host Format Antibody Type
    Ch, Xn, H, Yeast (S. cerevisiae ) Chromatin Immunoprecipitation (ChIP), WB Rabbit Purified Polyclonal Antibody
    Description:
    Anti-Histone H2B
    Background Information:
    Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.
    Alternate Names:
    • H2B histone family, member Q
    • histone 2, H2be
    • histone cluster 2, H2be
    Specificity:
    Recognizes and is specific for Histone H2B, MW ~15 kDa. An additional band at ~23 kDa is detected in some samples, which likely corresponds to ubiquityl-Histone H2B.
    Key Applications:
    • Chromatin Immunoprecipitation (ChIP)
    • Western Blotting
    Application Notes:
    Chromatin Immunoprecipitation (ChIP):
    A previous lot of this antibody was used in ChIP.
    Species Reactivity:
    • Chicken
    • Xenopus
    • Human
    • Yeast (S. cerevisiae )
    Species Reactivity Note:
    Human, chicken, yeast and Xenopus. Others not tested. Broad species cross-reactivity expected based on sequence homology.
    Isotype:
    IgG
    Immunogen:
    KLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CG-AVTKYTSSK) of human Histone H2B, with an N-terminal CG added for conjugation purposes.
    Molecular Weight:
    ~15 kDa
    Control:
    Acid extract from either sodium butyrate or colcemid treated HeLa cells.
    Presentation:
    Purified rabbit polyclonal in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide, and 30% glycerol.
    Storage Conditions:
    Stable for 1 year at -20ºC from date of receipt.
    Gene Symbol:
    • GL105
    • H2B
    • H2B-GL105
    • H2B.1
    • H2B.q
    • H2B/q
    • H2BFQ
    • MGC119802
    • MGC119804
    • MGC129733
    • MGC129734
    • OTTHUMP00000013920
    Quality Assurance:
    Evaluated by western blot on acid extracts from either sodium butyrate or colcemid treated HeLa cells.

    Western Blot Analysis:
    A 1:1,000-1:10,000 dilution of this antibody detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells (Catalog # 13-113 and 13-114 respectively), chicken core histone (Catalog # 13-107) and recombinant human Histone H2B (Catalog # 14-491). Recombinant Xenopus Histone H2B (Catalog # 14-410) was used with a previous lot.
    Antibody Category:
    Epigenetics & Nuclear Function
    Antibody Sub-Category:
    Histones
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Upstate (Millipore)
    Concentration:
    1 mg/mL
    Antibody Type:
    Polyclonal Antibody
    Format:
    Purified
    Host:
    Rabbit

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    peptide corresponding to amino acids 118-126 (CG-AVTKYTSSK) of human Histone H2B 详细描述见链接:http://www.millipore.com/catalogue/item/07-371

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    图标文献和实验
    相关实验
    • Histone Acetylation and Deacetylation

      transcription (1 ,2 ). DNA is packaged into chromatin, a highly organized and dynamic protein-DNA complex. The fundamental subunit of chromatin, the nucleosome, is composed of an octomer of four core histones, an H3/H4 tetramer and two H2A/H2B dimers, surrounded

    • 组蛋白 Histone Protein

      组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白;在细胞正常 pH 值时,组蛋白带有正电荷,这样它们就可以和 DNA 结合,这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实,组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg , Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白: H1 , H2A , H2B , H3

    • 组蛋白(histone)

      组蛋白是真核生物染色体的基本结构蛋白, 是一类小分子碱性蛋白质, 有五种类型:H1 、H2A 、H2B 、H3 、H4,它们富含带正电荷的碱性氨基酸, 能够同DNA中带负电荷的磷酸基团相互作用。 组蛋白的基因非常保守。亲缘关系较远的种属中, 四种组蛋白(H2A、H2A、H3、H4)氨基酸序列都非常相似, 如海胆组织H3的氨基酸序列与来自小牛胸腺的H3的氨基酸序列间只有一个氨基酸的差异, 小牛胸腺的H3的氨基酸序列与豌豆的H3也只有4个氨基酸不同。不同生物的H1序列变化

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