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- 24865-1 mg
- 2026年04月15日
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文献和实验by microcentrifugation at ≥ 8000 x g for 1-2 minutes.25.Repeat steps 23. and 24. This is a total of two washes with 1 mL PBS-TBN.26.Remove the supernatant and resuspend the coupled and washed microspheres in 250-1000 μL of PBS-TBN. 27.Count the microsphere
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
, except resuspend the cells (~8-10 x 105 cells), in 2 mL of freezing medium for OP9 cells per 10-cm dish. Preferably, freeze cells within the first two to three passages. 14. Aliquot 1 mL of cell suspension per cryovial. 15
the stock uncoupled microsphere suspension according to the instructions described in the Product Information Sheet provided with your microspheres. 2.Transfer 5.0 x 106 of the stock microspheres to a USA Scientific microcentrifuge tube.
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