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文献和实验Rinse with PSA 3x 0.3% hydrogen peroxide in PBS, 30min. Then rinse in PBS 3x 5% normal goad serum in PBS at RT for 1 hour. Rabbit anti-Human Factor VIII Related Antigen diluted to 1:500 with 1% NGS in PBS, incubated
Antigen Design Sera Purification Tech Sheet
, it is important to avoid self-recognition of the immunogen by the host animal. As an example, when raising antibodies against a human protein, it is more suitable to use a rabbit or mouse host than a monkey. For highly conserved mammalian proteins, raising
Antigen Design Sera Purification Tech Sheet
. In order to achieve maximum immune response, it is important to avoid self-recognition of the immunogen by the host animal. As an example, when raising antibodies against a human protein, it is more suitable to use a rabbit or mouse host than a monkey
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