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文献和实验Site-Directed Mutagenesis in the Investigation of -Adrenoreceptor Exosite
, such as metaproterenol, fenoterol, and terbutaline; and saligenins, such as salbutamol and salmeterol. Of these, salmeterol, a highly lipophilic derivative of the partial agonist, salbutamol, represents the latest long-acting generation of β-agonists, with a duration
我要做酵母双杂交,有战友在做吗,交流一下,我的QQ446357706,现在我先把我的资料给大家看看吧,我自己写的程序酵母培养:YPD: 蛋白胨20g ,酵母提取物10g ,琼脂20g葡萄糖20g或灭菌后加入葡萄糖(40% 50ml;过滤除菌/121℃,15min),使终浓度为2%YPDA: YPD+adenin hemisulfate(0.2% 15ml;对热不稳定,不能高温高压灭菌),使终浓度为0.003%.SD /:(SD /完全,SD /-./././.)无氨基酸酵母氮源 6.7g
Adenine hemisulfate* 2.0 gm Arginine HCl 2.0 gm Histidine HCl* 2.0 gm Isoleucine 2.0 gm Leucine* 2.0 gm Lysine HCl 2.0 gm Methionine 3.0 gm Phenylalanine 6.0 gm Homoserine 3.0 gm Tryptophan* 2.0 gm Tyrosine 1.2 gm Uracil
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