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文献和实验useful properties of full phosphoproteins. However, such pTyr-containing peptides have limited use in whole-cell systems resulting from lability of the phosphate ester bond to protein-tyrosine phosphatases (PTPs). For this reason, a number of phosphatase
-inflammatory drugs such as diclofenac did not only suppress the prostaglandin but also the F2 -IsoP pathway. Microdialysis allows the “near-in vivo” measurement of prostanoid mediators, released in the interstitial space of the dermis under inflammatory
Enzyme Immunoassay of Isoprostanes
, and excreted. It is now well recognized that isoprostane levels, most commonly those of 15F2t -isoprostane (formerly denoted 8-iso-prostaglandin F2 α ) in blood or urine, are useful biomarkers for the assessment of oxidative stress, which is a major risk factor
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