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文献和实验The MIRA Method for DNA Methylation Analysis
methylation patterns. In this chapter, we describe the methylated-CpG island recovery assay (MIRA), which is based on the high affinity of the MBD2b/MBD3L1 complex for double-stranded CpG-methylated DNA. MIRA has been used in combination with microarray
Methylated-CpG Island Recovery Assay
(MIRA), based on the high affinity of the MBD2b/MDB3L1 complex for double-stranded methylated DNA, allows for the recovery of methylated DNA without the use of bisulfite conversion or antibody recognition. MIRA is capable of detecting low-density
Immunofluorescent Staining of Drosophila Larval Brain Tissue
apical polar localization of aPKC (green) and basal polar localization of Miranda (Mira; blue). The mitotic spindle is visualized with anti- -tubulin (Tub; red). (B ) During telophase, apical proteins such as aPKC (green) are retained in the neuroblast
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