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文献和实验Preparation of Polyclonal Antibodies to Retinoid Receptors
of a retinoid “X” receptor α (RXRα) antibody on Western blots contributed to the identification of the RXR ligand by detection of the receptor in fractions of whole-cell lysates that were able to bind the 9-cis retinoic acid ligand (2 ) Antibodies
Restriction endonuclease digestion A six-cutter (e.g. Eco RI) will cut on average every 4.1 Kb . Complete digestion of human DNA with this type of enzyme will result in approximately 1 x 106 unique fragments. What is the probability
then serially diluted in 100 µl assay buffer on the plate). Incubate @ RT for 1 hr while shaking. Meanwhile let TMB solution equilibrate to RT. Wash plate 3x and add 100 µl/well rabbit polyclonal anti-p27 antibody diluted 1:1000 in assay buffer
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