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文献和实验Lac-Z Detection in Tail Biopsies
2mm tail biopsies are collected in 1X PBS (see recipes below) in a 1.5ml microfuge tube. Be sure to include a known +/+ (lac-Z negative) biopsy as a control. Replace 1X PBS solution over biopsy with 2.5% Glutaraldehyde diluted in SpM buffer
Staining Methods for cell death Z. Xia 10/2/95
The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluorescein diacetate)-green, alive cells; P.I. (propidium iodide)-red, dead cells 35 mm plates: to 2 ml medium or PBS, add 2 ul 2 mg/ml P
E.Z.N.A.® Protocol for Bacteria
实验试剂 Additional materials to be supplied by user 1. RNase-free Lysozyme 2. TE buffer (10 mM Tris-HCl, pH 7.6, 1 mM EDTA) Procedure 实验步骤 1. Harvest Cells and resuspend in 500 ul TE
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