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文献和实验in acetonitrile, dimethylformamide (DMF), or dimethylsulfoxide (DMSO); or 0.1 to 10 mM sulfosuccinimidyl ester reagent in H 2 O Protein solution: 0.1 to 2 mM protein in 0.1 M sodium bicarbonate, pH
Metabolic Labeling with Fatty Acids
of radiolabeled fatty acids (e.g., [3 H]myristate or palmitate) is an alternative method for labeling proteins. This unit contains methods for biosynthetic labeling with fatty acids, analysis of the fatty acid linkage with protein, analysis of total protein?bound
Immunohistochemisty-Fluorescence Protocol
, store at 4°C in the dark; prepare working dilution just prior to fixation Wash Buffer Earls Buffered Salt Solution (EBSS) (Gibco BRL) Wash Buffer-Saponin EBSS with 0.1 (w/v) Saponin (Sigma Catalog # S4521) and adjust pH to 7.2-7.4 with NaOH
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