Caspase-3/7 Fluorescence Assay

Cayman的Caspase-3/7荧光检测试剂盒采用特定底物N-Ac-DEVD-N'-MC-R110，该底物在被活性caspase-3或caspase-7切割后，会产生强荧光产物，可使用激发波长和发射波长分别485和535nm进行测量。该试剂盒使用简单，可轻松适应高通量筛选调节caspase-3或-7活化的治疗化合物。试剂盒中包含活性caspase-3，可用作阳性对照。试剂盒中还包含caspase-3/7抑制剂N-Ac-DEVD-CHO，用于验证底物的特异性。Cayman's Caspase-3/7 Fluorescence Assay Kit employs a specific substrate, N-Ac-DEVD-N'-MC-R110, which upon cleavage by active caspase-3 or caspase-7, generates a highly fluorescent product that can be measured using excitation and emission wavelengths of 485 and 535 nm, respectively. The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds regulating activation of caspase-3 or -7. Active caspase-3 is included in the kit for use as a positive control. The caspase-3/7 inhibitor, N-Ac-DEVD-CHO, is also included in the kit for verifying the specificity of the substrate.