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文献和实验Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins
-intensive. Here we describe a workflow for purification, characterisation and identification of DNA-binding proteins. We show the use of a fluorescence-based electrophoretic mobility shift assay (fEMSA) and describe its advantages for a rapid
Analysis of DNA Binding Proteins by Mobility Shift Assay
binding proteins. These include the gel mobility shift assay (1 ,2 ), DNase I footprinting analyses (3 ), and the methylation-interference assay (4 ,5 ). Although each technique can be used to obtain valuable information regarding DNA/protein
-binding site contributes to poor inhibitor selectivity, issues which have particularly hampered the development of novel kinase inhibitors. We developed a high-throughput screening technology that can discriminate for inhibitors which stabilize
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