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文献和实验, serine, and inositol. Phospholipid structures are designated by a stereospecific nomenclature (sn ) based on L-glycerol-3-phosphate. Thus, the fatty acid esterified to carbon-l of the glycerol backbone is termed the “sn-l ” fatty acid, and is commonly
down 5',5K,4℃ **wash means complete resuspention to remove all salt 6.Invert tube to drain SN ~5sec (soft plt).with p200,resuspend cells in remaining SN & transfer to eppendorf.Wash SS34 tube with another 100μl 10% glycerol and pool with cells.Vf ~300-500μl
Human Plasma Platelet-Activating Factor Acetylhydrolase
The human plasma platelet-activating factor acetylhydrolase (PAF acetylhydrolase, l-alkyl-2-acetyl-glycerophosphocholine esterase, l-alkyl-2-acetyl- sn -glycero-3-phosphocholine acetohydrolase, EC 3.1.1.47) is a phospholipase A 2 activity
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