Propidium Iodide Solution

PI染料：PI与双链DNA和RNA结合，在细胞膜通透化后，结合到核酸中，核呈红色，可用荧光显微镜观察，采用罗丹明滤光片进行观察。PI用于多色荧光染色分析，其鲜红色的核染色使其成为与FITC、PE或Cy5一起使用的优秀对比染料。在流式细胞术分析中，PI染色可以通过FL2通道监测。PI也可用于测量细胞活力，因为该染料不进入活细胞。PI binds to double-stranded DNA and RNA after cells have been permeabilized. Once bound to nucleic acids, the nuclei appear red in color as observed by fluorescence microscopy with a rhodamine filter. PI is useful for multi-color fluorescent staining analysis. The bright red staining of nuclei makes this an excellent counter stain to be used simultaneously with FITC, PE, or Cy5. For analysis by flow cytometry, PI staining can be monitored in the FL2 channel. PI can also be used to measure cell viability since this dye is excluded from viable cells.