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文献和实验NOTE - Use arachidonic acid from Biomol (# FA-003) 1) Make up the appropriate concentration of AA in 100% ethanol. 2) Add 1µl of phenol red solution. --> The solution should appear yellow since the free acid
with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin). --> "Appropriate" = bring cells to a final concentration of 5 X 105 cells/ml. 4) Add [3H] arachidonic acid to get ~2 µCi/ml (2 X 106 µCi/ml???). --> Make sure final AA
Products of Arachidonic Acid Metabolism
Segmentation of the nephron relative to transport mechanisms and secretory activity has been recognized for decades, beginning with the pioneering studies of Alfred Newton Richards (1 ). Nephron segmentation regarding transcellular sodium
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