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文献和实验Preparation and purification of biotin-labeled probe for ISH
product. The best ISH results are obtained when the size of the biotin-labeled probe is around 300-600 bp. To determine the size of the probe, denature 15 µl of the nick translation product in boiling water for 4 min and cool down on ice immediately
Detection of Specifically Oxidized Apolipoproteins in Oxidized HDL
Atherosclerosis is associated with dysfunctional HDL, and oxidation of HDL is thought to give rise to HDL becoming dysfunctional. Lipoprotein oxidation represents a complex series of processes that can be assessed by various methods
4-Hydroxynonenal (4-hydroxy-2,3-trans-nonenal; 4-HNE) is the best known and thoroughly studied aldehydic product originating in biological samples during the process of lipid peroxidation (Fig. 1 ) ( 1 ). The latter is an autocatalytic, self
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