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文献和实验Production of Polyclonal Antisera
All immunochemical procedures require a suitable antiserum or monoclonal antibody raised against the antigen of interest. Polyclonal antibodies are raised by injecting an immunogen into an animal and, after an appropriate time, collecting
Preparation of Polyclonal Antibodies to Retinoid Receptors
with antibodies to retinoic acid receptor a (RARα) enabled visualization of the promyelocyte-retinoic acid receptor (PML-RAR) fusion oncoprotein in cells from acute promyelocytrcleukemia patients (5 , 6 ) This chapter describes basic protocols for polyclonal
Polyclonal and Antigen-specific Responses of T Cells and T Cell Subsets
of polyclonal or antigen-specific responses in enriched or purified T cells, free of the influence of previously ethanol exposed accessory cells. In this chapter, we describe protocols to assess the T cell response to polyclonal stimulation through the T cell
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