- 询价
- 06-896
- 2025年07月10日
- Western Blotting;Immunocytochemistry
- Chicken
- Vertebrates
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- 详细信息
- 询价记录
- 技术资料
- 应用范围：
Western Blotting;Immunocytochemistry
- 宿主：
Chicken
- 适应物种：
Vertebrates
- 抗原来源：
0
- 库存：
大量
- 是否单克隆：
2
- 规格：
200 μg
Product Images
WB: rat brain lysate containing 25ng of GFP was probed with 1μg/ml of anti-GFP
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | IC, WB | Chicken | Purified | Polyclonal Antibody |
Description:
Anti-GFP (Green Fluorescent Protein)
Background Information:
Green fluorescent protein (GFP) is a 29 kDa fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488 nm), GFP strongly fluoresces (Emission max: 507 nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells.
Alternate Names:
Green fluorescent protein
Specificity:
Recognizes green fluorescent protein (GFP), Mr 30 kDa, and GFP-fusion proteins.
Key Applications:
- Immunocytochemistry
- Western Blotting
Application Notes:
Immunocytochemistry:
This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein.
This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein.
Species Reactivity:
Vertebrates
Species Reactivity Note:
Vertebrates
Isotype:
IgY
Immunogen:
His-tagged green fluorescent protein of Aequorea victoria.
Molecular Weight:
30 kDa
Control:
Rat brain lysate, cells expressing GFP protein.
Presentation:
Purified chicken polyclonal IgY from egg yolk in buffer containing 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Gene Symbol:
GFP
Quality Assurance:
Routinely evaluated by Western Blot on rat brain lysate containing purified GFP (Catalog #14-392) or a GFP-fusion protein from a transfected E. coli cell lysate.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate.
Antibody Category:
Epitope Tags & General Use
Antibody Sub-Category:
Epitope Tags
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Purified
Host:
Chicken
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
His-tagged green fluorescent protein of Aequorea victoria 详细描述见链接：http://www.millipore.com/catalogue/item/06-896
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Anti-GFP (Green Fluorescent Protein); 200 μg
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