- 询价
- 06-896
- 2025年07月10日
- Western Blotting;Immunocytochemistry
- Chicken
- Vertebrates
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 应用范围：
Western Blotting;Immunocytochemistry
- 宿主：
Chicken
- 适应物种：
Vertebrates
- 抗原来源：
0
- 库存：
大量
- 是否单克隆：
2
- 规格：
200 μg
Product Images
WB: rat brain lysate containing 25ng of GFP was probed with 1μg/ml of anti-GFP
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Vrt | IC, WB | Chicken | Purified | Polyclonal Antibody |
- Immunocytochemistry
- Western Blotting
This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate.
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His-tagged green fluorescent protein of Aequorea victoria 详细描述见链接：http://www.millipore.com/catalogue/item/06-896
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- 作者
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- 询问日期
文献和实验Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kDa protein absorbs blue light from a photoprotein that is activated by calcium and emits green
await to be uncovered. We recently developed a modular two-plasmid system, based on the green fluorescent protein (GFP) as non-invasive reporter of gene expression, to rapidly monitor the regulatory potential of sRNA/target mRNA pairs under investigation
Anti-GFP/BrdU In Situ Immunostaining
by adding 1 mL PBS, 3' each. Add 0.3 mL rabbit anti-GFP (1:200 v/v, plus 1% goat serum in PBS) and incubate 1 hr. at room temp. Wash 3x in PBS. Post-fix in 1 mL 4% paraformaldehyde x15', room temp. Wash 3x in PBS. Denature DNA with 1 mL
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