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文献和实验saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
Fusion of Mouse, Rat, or Hamster Cell
the pellet in HAT medium to 5 x 105 cells/ml. Plate 200 ml/well to 96 well plates. Incubate at 37o C, 8-10 CO2 for 5-7 days.
Transgenic Mouse and Transgenic Rat Outline
into fertilized mouse or rat eggs, the Transgenic Core requires that you provide evidence that you have a PCR or Southern blot assay that detects your transgene when it is spiked into tail DNA at a one copy concentration. A second assay that will detect
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