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文献和实验1). Albumin or powdered milk is used to block the remaining sites which could result in false positives. Monoclonal antibodies will react with the antigen attached to the microtiter plate. The complex antigen-antibody is then reacted with enzyme conjugates
-CoV-2 emerge and spread epidemically around the world, serologic testing is essential to identify currently infected or potentially immune individuals1 . In addition, serological assays using the antigen-down format are routinely used in autoimmunity
of interest; the same antigen with which the previous plate has been sensitized. The negative control is PBS). Do not use the outermost wells of the plate, as these have not been sensitized in the previous plate. See diagram. 2.Pipette 60 µl of 5% skim milk/PBS/Tween
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