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文献和实验Primary Cultures of Astrocytes from Fetal Bovine Brain
differentiation and the prevalence of glial cells vs. neurons. We compare explants from early, middle, and late stages of development and two different fetal calf serum concentrations (1 and 10%) to identify the best conditions to obtain
Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Isolation of Microtubules (Bovine Brain)
content of your sample using the Bradford protein assay ( Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard curve. Decant the remainder of the crude supernatant into a chilled beaker, and add an equal
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