- 询价
- 05-753
- 2025年07月10日
- Western Blotting;Immunocytochemistry
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Western Blotting;Immunocytochemistry
- 宿主：
Mouse
- 适应物种：
Human
- 抗原来源：
0
- 库存：
大量
- 是否单克隆：
1
- 规格：
200 μg
6His-tagged fusion protein corresponding to residues 1-181 of human WT1 (Wilms tumor) 详细描述见链接：http://www.millipore.com/catalogue/item/05-753
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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
X75 mm Falcon tube containing 3 ml of LB medium (10 g Bacto-Tryptone, 5 g Bacto-yeast extract, and 10 g NaCl in 1 L H2O, autoclaved) with appropriate antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture
Clone Genes From a Phage Library
much more complicated than it actually is. First you must have the SolR cells grown up and prepared for phage plating, and get out some Exassist phage as well from the freezer. • In a 15 ml falcon tube, combine 200 microliters XL blue cells and 200
1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
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