产品简介
Annexin V(或 Annexin A5)为胞内蛋白膜联蛋白家族成员,以钙依赖的方式与磷脂酰丝氨酸(PS)结合。PS 存在于正常细胞浆膜的内层,但在凋亡早期,膜不对称性丧失,PS易位至细胞表面。荧光标记的 Annexin V 可与之特异性结合,表明该细胞为凋亡细胞。
产品特点
- 操作简便,15分钟即可完成染色;
- 分群清晰,结果稳定可靠;
- 独家搭配阳性质控液,补偿调节不再困难。
结果示例

试剂盒组分

Annexin V-PE/7-AAD Apoptosis Kit
Annexin V-PE/7-AAD Apoptosis Kit
AP104
100T
细胞凋亡试剂盒
100T
Annexin V(或 Annexin A5)为胞内蛋白膜联蛋白家族成员,以钙依赖的方式与磷脂酰丝氨酸(PS)结合。PS 存在于正常细胞浆膜的内层,但在凋亡早期,膜不对称性丧失,PS易位至细胞表面。荧光标记的 Annexin V 可与之特异性结合,表明该细胞为凋亡细胞。


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文献和实验1
Zhang J, Wang Y, Li G, et al. Down-Regulation of Nicotinamide N-m ethyltransferase Induces Apoptosis in Human Breast Cancer Cells via the Mitochondria-Mediated Pathway[J]. PLOS ONE, 2014, 9(2): e89202 (IF:3.73)
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2
Xie X, Yu H, Wang Y, et al. Nicotinamide N-m ethyltransferase enhances the capacity of tumorigenesis associated with the promotion of cell cycle progression in human colorectal cancer cells[J]. Archives of Biochemistry and Biophysics, 2014, 564: 52-66 (IF:3.043)
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3
Fu X, Gao H, Tian F, et al. Mechanistic Effects of Amino Acids and Glucose in a Novel Glutaric Aciduria Type 1 Cell Model[J]. PLOS ONE, 2014, 9 (IF:3.057)
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4
Wu D, Zhang J, Wang J, et al. Hesperetin induces apoptosis of esophageal cancer cells via mitochondrial pathway mediated by the increased intracellular reactive oxygen species[J]. Tumor Biology, 2015: 1-9 (IF:3.611)
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5
Chen S S, Hu W, Wang Z, et al. p8 attenuates the apoptosis induced by dihydroartemisinin in cancer cells through promoting autophagy[J]. Cancer Biology & Therapy, 2015, 16(5): 7779 (IF:3.072)
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6
Li L, Liu H C, Wang C, et al. Overexpression ofr, βr, -Catenin Induces Cisplatin Resistance in Oral Squamous Cell Carcinoma[J]. BioMed Research International, 2016: 1-11 (IF:2.476)
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7
Xu Y, Zhang Z, Li J, et al. The ubiquitin-conjugating enzyme UBE2O modulates c-Maf stability and induces myeloma cell apoptosis[J]. Journal of Hematology & Oncology, 2017, 10(1): 132 (IF:6.35)
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择? A:第一步考虑流式细胞仪配置,第二步考虑细胞自发荧光。对于本身有荧光的细胞(如转染了荧光蛋白基因的细胞或者药物孵育的细胞),特别是培养时间久或药物处理的细胞,一定要注意细胞代谢物和药物的荧光。一般来说,细胞代谢物的荧光会干扰 FITC/PE,所以建议用 Annexin V-APC,药物的干扰可查看药物本身的发射光谱是否会对检测选择的染料有影响。 Annexin V-APC 与 7-AAD 两种染料之间补偿干扰很小,染色操作简单,实验准确。 7-AAD 染料相比于 PI 染料粘稠度小,可减少检测
产品名称 规格 Annexin V-FITC Apoptosis Detection Kit I 100tests 产品组成成分: Annexin V Binding Buffer, 10X conc (51-66121E) Annexin V-FITC (51-65874X
A general protocol for staining cell for cytometry analysis
V-FITC (Cat. No. 65874X, 65874H) or Annexin V-Biotin (Cat. Nos. 65872X, 65872H). 3. Via-Probe™ (Cat. No. 34321X): A convenient ready-to-use solution of the nucleic acid dye 7-AAD. Recommended for use in parallel with Annexin V-PE (Cat
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