延胡索酸检测分析试剂盒

Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Application- Kit can detect as low as 1 nmol of fumarate per well (20 µM).

Features & Benefits:
• Simple procedure; takes ~ 40 minutes
• Fast and convenient

Kit components:
• Fumarate Assay Buffer
• Fumarate Enzyme Mix
• Fumarate Developer
• Fumarate Standard (0.1 M)

Description:
Fumarate (HO₂CCH=CHCO₂H-) is an intermediate in the Kreb’s cycle used by cells to metabolize food to form ATP. In the mammalian liver, Fumarate is also a product of the Urea cycle where its release in the cytosol leads to its conversion into malate and subsequently oxaloacetate while generating NADH in the cytosol. The human skin naturally produces fumaric acid when exposed to sunlight. In fact, fumaric acid esters have been used to treat psoriasis, possibly due to an impaired production of fumaric acid in the skin. Fumaric acid has also been used in beverages, baking powders and candy. BioVision’s Fumarate Assay Kit provides a convenient tool for sensitive detection of the fumarate in a variety of samples. The fumarate Enzyme Mix recognizes fumarate as a specific substrate leading to proportional color development. The amount of fumarate can therefore be easily quantified using a colorimetric assay (λ = 450 nm). It can detect as low as 1 nmol of fumarate per well (20 µM).

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.