Mouse单克隆Akt/PKB抗体 人鼠大鼠适用 akt单克

Anti-Akt/PKB, PH Domain, clone SKB1

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

SKB1

Recognizes Akt1/PKBα.

• Flow Cytometry
• IP-Kinase Assay
• Immunocytochemistry
• Immunoprecipitation
• Western Blotting

• Human
• Mouse
• Rat

IgG

GST-fusion protein corresponding to residues 1-149 of human Akt1

60 kDa

Positive Control Included: Non-Stim A431 Cell Lysate (12-301)

Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.02 M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide and 30% glycerol.

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

• AKT1
• RAC
• PRKBA
• MGC99656
• RAC-ALPHA
• RAC-PK-alpha
• C-AKT
• PKB
• AKT

routinely evaluated by immunoblot on RIPA lysates from A431 cells

Signaling

PI3K, Akt, & mTOR Signaling

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Upstate (Millipore)

1 mg/mL

Monoclonal Antibody

Purified

Mouse