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Anti-FGF-2 basic FGF单克隆抗体 小鼠源多

物种适用 bfgf抗体
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  • 询价
  • 05-118
  • 2025年07月15日
  • Radioimmunoassay;Western Blotting
  • Mouse
  • Bovine;Human;Mouse;Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      Radioimmunoassay;Western Blotting

    • 宿主

      Mouse

    • 适应物种

      Bovine;Human;Mouse;Rat

    • 库存

      大量

    • 抗原来源

      0

    • 是否单克隆

      1

    • 规格

      500 μg

    Product Images

    WB: FGF-2 probed with anti-FGF-2 at 1μg/ml

    Species Reactivity Key Applications Host Format Antibody Type
    H, B, M, R IH, RIA, WB Mouse Purified Monoclonal Antibody
    Description:
    Anti-FGF-2/basic FGF, clone bFM-2
    Background Information:
    FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
    Alternate Names:
    • Basic fibroblast growth factor
    • basic fibroblast growth factor bFGF
    • fibroblast growth factor 2
    • fibroblast growth factor 2 (basic)
    • heparin-binding growth factor 2
    Clone:
    bFM-2
    Specificity:
    This antibody detects FGF-2/basic FGF.
    Key Applications:
    • Immunohistochemistry
    • Radioimmunoassay
    • Western Blotting
    Application Notes:
    Immunohistochemistry:
    Previous lots of this antibody have been shown to immunostain frozen brain sections fixed with ice-cold, ethanol:acetic acid [95:5]. This antibody has also been used at 10 μg/mL to stain paraffin-embedded sections (Matsuzaki, K., 1989).
    Species Reactivity:
    • Human
    • Bovine
    • Mouse
    • Rat
    Species Reactivity Note:
    This antibody is highly specific for basic FGF from bovine, human, rat, and mouse sources and does not cross-react with bovine acidic FGF (FGF-1, HBGF-1).

    Important Note: This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity (Uteza, Y., 1999). Upstate recommends anti-FGF-2/basic FGF, clone bFM-1 (neutralizing) (Catalog # 05-117) for neutralizing activity.
    Isotype:
    IgG1κ
    Immunogen:
    Purified bovine brain basic FGF (FGF-2, HBGF-2). Clone bFM-2.
    Molecular Weight:
    17.5 kDa
    Control:
    Fetal liver or kidney tissue.
    Presentation:
    Purified mouse monoclonal IgG1κ in buffer containing PBS, pH 7.4, purified by ammonium sulfate precipitation and DEAE Sephacryl chromatography. Frozen solution.
    Storage Conditions:
    Stable for 1 year at -20ºC from date of receipt.
    Gene Symbol:
    • FGF2
    • Prostatropin
    • BFGF
    • prostatropin
    • HBGF-2
    • FGFB
    Quality Assurance:
    Evaluated on basic FGF (Catalog #01-114).

    Western Blot Analysis:
    0.5-2 μg/mL of this antibody detected 100 ng FGF-2/basic FGF, carrier-free (Catalog # 01-114).
    Antibody Category:
    Signaling
    Antibody Sub-Category:
    Growth Factors & Receptors
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Upstate (Millipore)
    Concentration:
    1 mg/mL
    Antibody Type:
    Monoclonal Antibody
    Format:
    Purified
    Host:
    Mouse
    Product Overview:
    This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity. Upstate recommends anti-FGF-2/basic FGF, neutralizing (Catalog #05-117) for neutralizing activity.

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    Purified bovine basic FGF 详细描述见链接:http://www.millipore.com/catalogue/item/05-118

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    图标文献和实验
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    • 抗体指南:你知道做什么实验用什么抗体吗?

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    • 抗体选择指南

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