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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Radioimmunoassay;Western Blotting
- 宿主:
Mouse
- 适应物种:
Bovine;Human;Mouse;Rat
- 库存:
大量
- 抗原来源:
0
- 是否单克隆:
1
- 规格:
500 μg
Product Images
WB: FGF-2 probed with anti-FGF-2 at 1μg/ml
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, B, M, R | IH, RIA, WB | Mouse | Purified | Monoclonal Antibody |
- Basic fibroblast growth factor
- basic fibroblast growth factor bFGF
- fibroblast growth factor 2
- fibroblast growth factor 2 (basic)
- heparin-binding growth factor 2
- Immunohistochemistry
- Radioimmunoassay
- Western Blotting
Previous lots of this antibody have been shown to immunostain frozen brain sections fixed with ice-cold, ethanol:acetic acid [95:5]. This antibody has also been used at 10 μg/mL to stain paraffin-embedded sections (Matsuzaki, K., 1989).
- Human
- Bovine
- Mouse
- Rat
Important Note: This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity (Uteza, Y., 1999). Upstate recommends anti-FGF-2/basic FGF, clone bFM-1 (neutralizing) (Catalog # 05-117) for neutralizing activity.
- FGF2
- Prostatropin
- BFGF
- prostatropin
- HBGF-2
- FGFB
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected 100 ng FGF-2/basic FGF, carrier-free (Catalog # 01-114).
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
Purified bovine basic FGF 详细描述见链接:http://www.millipore.com/catalogue/item/05-118
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文献和实验Purification of Growth Factor mRNA in Renal Tissues:bFGF-2, FGF-2, TGFα, and EGFR
(2 ). These heparin-binding factors are categorized as FGF-1 through FGF-10. Acidic FGF, or FGF-1, is found mostly in brain and other neural tissues. Basic FGF, or FGF- 2, a protein of 18 kDa mw, is one of the most ubiqitous growth factors. It is found in numerous
出现IgG 分子50 and 25 kDa 的重链和轻链条带。 5 二抗的选择 二抗应选用与使用的一抗相同的物种来源,例如:如果你的一抗是小鼠的单克隆抗体,二抗则选抗小鼠的二抗anti-mouse secondary。建议检查二抗说明书确保该抗体适用于你的检测应用, 二抗一般连接荧光素FITC 或发光团。 6 双重染色抗体的选择用未偶联一抗进行细胞培养物或组织切片的双重免疫染色要求一抗来源于不同物种并且二抗分别识别其中之一,二抗说明书应描述其与其它物种来源的免疫球蛋有否有交叉吸附。 想了解
条带。 五、 二抗的选择 二抗应选用与使用的一抗相同的物种来源,例如:如果你的一抗是小鼠的单克隆抗体,二抗则选抗小鼠的二抗anti-mouse secondary。建议检查二抗说明书确保该抗体适用于你的检测应用, 二抗一般连接荧光素FITC 或发光团。 六、双重染色抗体的选择 用未偶联一抗进行细胞培养物或组织切片的双重免疫染色要求一抗来源于不同物种并且二抗分别识别其中之一,二抗说明书应描述其与其它物种来源的免疫球蛋有否有交叉吸附。 七、荧光和化学发光标记 连接于二抗的标记
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