HepatiCult™ Organoid Growth Medium (Mouse) is a serum-free, defined cell culture medium for establishment and maintenance of mouse hepatic progenitor organoids. These organoids, or "mini-livers", provide an in vitro organotypic culture system for studying hepatic stem and progenitor cells. Organoids grown in HepatiCult™ feature an epithelium expressing genes marking hepatic stem and progenitor cells (PROM1, AXIN2, SOX9 and CD44), ducts (KRT19 and HNF1b) and hepatocytes (HNF4a, AFP). Hepatic organoids can be passaged every 4 - 7 days, can be cryopreserved, and are primed for downstream differentiation.
HepatiCult™ supports mouse hepatic organoid culture either embedded in Corning® Matrigel® domes or in a dilute Matrigel® suspension. Organoid culture enables convenient in vitro characterization of the hepatic epithelium in a physiologically relevant system and reduces the need for animal use.
Advantages: • Convenient in vitro system for generating organoids in 4 - 5 days • Step-by-step protocol with no injury models, hand-picking of ducts, or cell sorting required • Simple, two-component format; serum-free and defined medium formulation • Flexible protocol for generation and maintenance of organoid cultures in matrix domes or suspension culture
Components: • HepatiCult™ OGM Mouse Basal Medium, 95 mL • HepatiCult™ OGM Mouse Supplement, 5 mL
