The EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit is designed to isolate CD4+CD127lowCD49d- cells from fresh peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD4+ T cells, CD127high, CD49d+ cells and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. The desired cells are poured off into a new tube.
Advantages: • Fast, easy-to-use and column-free
• Up to 87.4% purity
• Isolated cells are untouched
Components:
EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232)
EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
EasySep™ Dextran Selection Cocktail, 1 mL
EasySep™ Magnetic Particles, 3 x 1 mL
RoboSep™ CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit (Catalog #19232RF)
EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Cocktail, 1 mL
EasySep™ Dextran Selection Cocktail, 1 mL
EasySep™ Magnetic Particles, 3 x 1 mL
RoboSep™ Buffer (Catalog #20104)
RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility: • EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)