【NusA】重组大肠杆菌转录终止/抗终止L因子/【NusA】

产品名称：【NusA】重组大肠杆菌转录终止/抗终止L因子-Recombinant E.Coli Transcription Termination/Antitermination L Factor

产品货号：PRO-623

产品规格：10ug///1mg///50ug

来源宿主：大肠杆菌中重组表达

保存建议：【NusA】重组大肠杆菌转录终止/抗终止L因子-Recombinant E.Coli Transcription Termination/Antitermination L FactorNusA although stable 4℃ for 4 weeks， should be stored desiccated below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.Please prevent freeze-thaw cycles.

背景资料：NusA is an important player in both prevention and enhancement of transcriptional termination. NusA is important both in Rho-dependent and intrinsic termination， as well as in lambda and other phage antitermination systems. The NusA gene was first identified by isolation of the nusAl mutation， which limits bacteriophage-l growth by preventing the antitermination activity of the l N protein. NusA plays a role in transcriptional antitermination in the cell. It has been shown to specifically aid in read-through of the RNA polymerase genes rpoB and rpoC， as well as in successful synthesis of the ribosomal RNA genes. Additionally to its anti-termination role， NusA is needed for both Rho-dependent and intrinsic transcriptional termination. NusA is obligatory for Rho-dependent termination in lambda phage and in the cell. NusA plays a role in intrinsic termination and the inhibition of RNA elongation. However NusA interacts with all three subunits of RNA polymerase， its termination activity primarily depends on its interaction with the carboxy-terminus of RpoA. NusA induces conformational change in RNA polymerase & prevents RNA interaction with RpoA. This binding sequentially activates NusA， allowing it to bind RNA and promote formation of hairpins at intrinsic termination sites. NusA binds Rho， and participates with sigma70 for binding to the core RNA polymerase complex. NusA does not compete with NusG for binding to either Rho or the polymerase， despite modulating the same process as NusG in both cases.

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关键词：Recombinant E.Coli Transcription Termination/Antitermination L Factor，【NusA】重组大肠杆菌转录终止/抗终止L因子，NusA