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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Escherichia coli Peptide chain release factor 1 (prfA)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Escherichia coli Peptide chain release factor 1 (prfA)品名简称:
Recombinant E.coli prfA protein货号:
CSB-EP015197ENV规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 95% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
prfAUniprot No.:
P0A7I0别名:
RF-1种属:
Escherichia coli (strain K12)蛋白长度:
Full Length来源:
E.coli分子量:
47.4 kDa表达区域:
1-360aa氨基酸序列:
MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQLSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLPKDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYKEIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDINPADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGARIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIEPIIQEHQADQLAALSEQE蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.功能2:
/文献:
/研究领域:
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文献和实验多肽链延伸因子 polypeptide chain elonga-tion factor
蛋白质生物合成中参与多肽链延伸过程的蛋白质因子。从大肠杆菌等原核细胞可纯化获得三种延伸因子(elongation factor),即EFTu,EFTs和EFG,分子量大约分别为4.7万,3.6万和8.3万。EFTu与GTP结合成EFTu-GTP,然后再与氨酰tRNA结合,形成三复合体(ternary complex)氨酰-tRNA-EFTu-GTP。这种三复合体与核糖体的氨酰tRNA部位(A部位,aminoacyl site)结合,继而GTP被分解,EFTu以EFTu-
多肽链终止因子 polypeptide chain releasefactor,olypeptide chain termination fa-ctor
参与蛋白质生物合成终止过程的蛋白质因子。是将在核糖体上刚刚合成的多肽链由tRNA 切断,使之游离的因子,亦称为 R 因子( release factor )。从大肠杆菌中分离出 RF 1, RF 2和 RF 3三种 R 因子。分子量大约分别为 4.4 万, 4.7 万和 4.8 万。 RF 1能识别终止密码子( termination codon ) UAG 、 UAA ; RF 2能识别终止密码子 UAA 和 UGA ,并能将其结合到核糖体上,由于 50S 亚基的肽
at the DNA level. Another factor affecting the success of peptide mass fingerprinting is mass accuracy (32, 62). Because it is critical to obtain an accurate measurement of the masses of multiple peptides, factors that alter the masses of those peptides
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